New release: MicroCT analysis of ameloblast specific Smad4 conditional knockout mice and Ambn-IRESCre mouse models
Published 11 February 2026
Mutant sample from the dataset MicroCT analysis of ameloblast specific Smad4 conditional knockout mice
Description:
Michael Paine’s lab (University of Southern California) developed a novel ameloblastin driven Cre-recombinase mouse line (Ambn-IRESCre MMRRC Strain #067446-JAX; RRID:MMRRC_067446-JAX) with Cre expression limited to enamel forming cells called ameloblasts. This is a significant advance from the current Cre mouse lines used in conditional gene silencing to study enamel formation. We used this Ambn-IRESCre line to knockdown the transcription factor Smad4 specifically in ameloblasts and achieved an extreme pathological phenotype limited to tooth enamel with no effects on the physiological functions of Smad4. These data highlight the significance of the Ambn-IRESCre mouse model for future studies defining gene functions during enamel formation.
Related Resource:
This data is part of EnamelBase, a community resource providing validated genetic mouse tools and multi-scale data to study gene expression, structure, and mechanical properties of developing and mature tooth enamel.
FaceBase Datasets:
Michael Lansdell Paine, Rucha Arun Bapat, Alexis Bauer, David Evans, Yanbin Ji, Marziyeh Aghazadeh. MicroCT analysis of ameloblast specific _Smad4_ conditional knockout mice. FaceBase Consortium https://doi.org/10.25550/2W-YN1C (2026).
Rucha Arun Bapat, Alexis Bauer, David Evans, Marziyeh Aghazadeh, Yanbin Ji, Michael Lansdell Paine. MicroCT analysis of Ambn-IRESCre mouse models. FaceBase Consortium https://doi.org/10.25550/7X-HZDA (2026).