EnamelBase: Protocols

Collectively, the four teams have developed a series of protocols that govern data collection in the following major areas:

Fixation and tissue processing

• Mouse Perfusion

• Tissue Processing for Histology Sectioning

• Formalin-Fixed Paraffin-Embedded (FFPE) Sample Preparation and Pretreatment

• Material and Supplies LM EM Histology

• Decalcification Protocol

Genotyping

• Genotyping Protocol for Odaph and Slc13a5 Mouse Models

• Confirmation of All Coding Exons and Exon-intron Junctions for Odaph and Slc13a5 Mouse Models

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Imaging of Soft Tissues

• RNAscope 2.5 HD Detection Reagent

• RNAscope Protocol

• Immunohistochemistry Protocol

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Analysis Crystallographic and Mechanical Properties

• Nanoindentation of Mouse Incisors

• Microbeam Diffraction of Mouse Incisors

• X-Ray Scattering (SAXS and WAXS) of Mouse incisors

• Mg K-edge X-Ray Absorption Near Edge Spectroscopy of Mouse Incisors

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Imaging and Compositional Analysis of Mineralized Tissues

• Synchrotron Parallel-Beam MicroComputed Tomography of Mouse Hemimandibles

• Scanning Electron Microscopy of Mouse Incisors

• Scanning Electron Microscopy-Energy Dispersive Spectroscopy of mouse incisors

• Confocal Raman Microscopy of Mouse Incisors

• Infrared Spectroscopy of Mouse Incisors

• Atom Probe Tomography of Mouse Enamel

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